Conjugate Pad Release: How Drying and Pretreatment Affect Test-Line Consistency
A weak or missing test line rarely starts at the test line. In most colloidal gold lateral flow assays, the real problem starts upstream, at the conjugate pad. When gold conjugate release fails or runs inconsistently, the strip cannot deliver a reliable signal no matter how well the nitrocellulose membrane or antibodies perform downstream. For […]
